In the Spider-Island storyline, Captain America is captured by the Jackal and forcibly mutated into a giant spider mutant, whom the Jackal calls "Spider-King."  As Spider-King, Steve Rogers is impregnated with thousands of spider eggs in order to spread the virus that gives people spider-powers. He is stopped and captured by the new heroic version of Venom, but he escapes captivity by vomiting out the now-hatched mutant spiders. Ultimately he is recaptured and put into suspended animation, while Venom disguises himself as Spider-King to infiltrate the Jackal's organization. 
When the cure for the Spider-Island virus was discovered, he was returned to normal and later joined Venom in his fight against the Queen, the true mastermind behind the virus. Venom stabbed her with Captain America's shield, an attack that should have killed her. Instead, she mutated into a 28-story spider monster. 
The two led her to Union Square, where they were joined by other heroes while Spider-Man distributed the cure to the infected New Yorkers. This weakened her enough for Kaine, Spider-Man's clone, to kill her.